Recombinant Histone H3.3

 

Catalog # : EPX-09-RH

 

Source : Human

 

Expressed in : E. Coli

 

Quantity : 100ug of recombinant histone H3.3 at 1ug /ul

 

 

 

Background:

 

The histone variant H3.3 replaces conventional H3.1 in a wide range of nucleosomes in active genes and constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis (1, 2).

 

 

Protein details:

 

Recombinant human histone H3.3 was produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM sodium phosphate pH 7.0, 1mM EDTA, 0.3 M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.

 

 

Quality control:

 

Each lot has been evaluated by ESI-TOF analysis and 12% SDS-PAGE (NuPAGE MOPS SDS, Invitrogen).

 

center  

SDS-PAGE gel of recombinant Histone H3.3 (Lane 2).

  ESi-TOF analysis of recombinant human histone H3.3.

 

Application Notes:

 

For research use only.

 

 

Storage:

 

-80°C

 

 

Guarantee:

 

Products guaranteed stable for 2 years from date of receipt when stored properly.

 

 

Purity:

 

>98% purity by SDS PAGE.

 

 

Protein sequences:

Human histone H3.3 (Theoretical Mw: 15196.72)

ARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGT VALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIG ALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

 

References:

1. Drané et al., (2010). Genes Dev. Jun 15;24(12):1253-65.

2. Ahmad and Henikoff. (2002). Mol Cell. Jun;9(6):1191-200.

 

 

 


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