Recombinant H3.3 Core Histones

Catalog # : EPX-05-RH

Source : Human

Expressed in : E. Coli

Quantity : 25ug of recombinant core histones at 1ug /ul

Background:

The histone Variant H3.3 replaces conventional histone H3.1 in a wide range of nucleosomes in active genes and is incorporated into chromatin independently of DNA synthesis (1). H3.3 core Histones are variant nucleosomal core components were H3.1 is replaced by H3.3 (2). The H3.3 variant nucleosome consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins H2A, H2B, H3.3 and H4 (3).

Protein details:

Recombinant human H3.3 core histones were produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM Tris-Cl pH 7.9, 1mM EDTA, 2M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.

Quality control:

Each lot has been evaluated by ESI-TOF analysis and 14% SDS-PAGE (NOVEX Tris-Glycine SDS, Invitrogen).

SDS-PAGE gel of human core Histones. 2ug of recombinant H3.3 core histones (lane 3) compared with recombinant H3.1 core histones (lane 2). Lane 1, protein molecular weight marker.		ESi-TOF analysis of recombinant human H3.3 core histones.

Application Notes:

For research use only.

Storage:

-80°C

Guarantee:

Products guaranteed stable for 2 years from date of receipt when stored properly.

Purity:

>98% purity by SDS PAGE.

Protein sequences:

H2A (Theoretical Mw: 14091.48)

MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGA

PVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNK

LLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

H2B (Theoretical Mw: 13904.17)

MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQ

VHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQ

TAVRLLLPGELAKHAVSEGTKAVTKYTSAK

H3.3 (Theoretical Mw: 15327.91)

MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVA

LREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEA

SEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

H4 (Theoretical Mw: 11367.34)

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRIS

GLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGR

TLYGFGG

References:

1. Ahmad and Henikoff. (2002). Mol Cell. Jun;9(6):1191-200.

2. Drané et al., (2010). Genes Dev. Jun 15;24(12):1253-65.

3. Luger et al., (1997) Nature, 389(6648):251-60.