Recombinant human Hp1β (CBX1)

Catalog # : EPX-002-REP

Source : Human

Expressed in : E. Coli

Quantity : 25µg of recombinant human Hp1β at 0.5µg /µl

Background:

Hp1β (CBX1) is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression (1). Hp1β do not associates with chromosomes during mitosis (2). Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane (3).

Protein details:

Recombinant human N-terminal His tagged Hp1β was produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM Tris-Cl pH 7.6, 1mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry. >98% purity by SDS-PAGE.

Quality control:

Each lot has been evaluated by 10% Tris Glycine SDS-PAGE.

SDS-PAGE gel of recombinant Hp1β (Lane 2).  Lane 1, protein molecular weight marker.

Storage:

-80°C

Guarantee:

For research use only. Products guaranteed stable for 2 years from date of receipt when stored properly.

Purity:

>98% purity by SDS PAGE.

Sequence:

Hp1β (Mw: 22,596 Da.)
MIHHHHHHLEGKKQNKKKVEEVLEEEEEEYV
VEKVLDRRVVKGKVEYLLKWKGFSDEDNTWE
PEENLDCPDLIAEFLQSQKTAHETDKSEGGK
RKADSDSEDKGEESKPKKKKEESEKPRGFAR
GLEPERIIGATDSSGELMFLMKWKNSDEADL
VPAKEANVKCPQVVISFYEERLTWHSYPSED
DDKKDDKN

References:

1.    Lachner et al., Nature 410:116-120(2001).
2.    Minc et al., Chromosoma 108:220-234(1999)
3.    Ye et al., J. Biol. Chem. 272:14983-14989(1997)