Recombinant human Hp1β (CBX1)
Catalog # : EPX-002-REP
Source : Human
Expressed in : E. Coli
Quantity : 25µg of recombinant human Hp1β at 0.5µg /µl
Hp1β (CBX1) is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression (1). Hp1β do not associates with chromosomes during mitosis (2). Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane (3).
Recombinant human N-terminal His tagged Hp1β was produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM Tris-Cl pH 7.6, 1mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry. >98% purity by SDS-PAGE.
Each lot has been evaluated by 10% Tris Glycine SDS-PAGE.
For research use only. Products guaranteed stable for 2 years from date of receipt when stored properly.
>98% purity by SDS PAGE.
Hp1β (Mw: 22,596 Da.)
1. Lachner et al., Nature